Single Cell Plant Model of Equisetum arvense for the Study Antihistamine Effects of Azulene and Sesquiterpene Lactones

Single cell of vegetative microspore from spore-bearing plant field Equisetum arvense L. has been presented as a single-cell experimental model for the screening native compounds acting antihistamine agents. The effects azulene, sesquiterpene lactones austricine, gaillardine, grosshemine, inulicine, and desacetylinulicine well alcohol ledol, on content histamine in germinating horsetail microspores investigated by fluorescent method. It shown using microspectrofluorimetry that these are able to regulate germination varying degrees, assessed autofluorescence chlorophyll, medium without presence 0.5–1% sodium sulfate salt stress factor. A histochemical reaction with ortho-phthalic aldehyde cells secretory mucilage revealed ability studied reduce level this biogenic amine depending their structure following order: grosshemine > azulene austricine ledol. Gaillardine, showed weak activity